Increased numbers of γδ lineage T cells in the absence of Maml1. (A) Flow cytometric analysis showed an increased percentage of TCRγ⁺ cells among CD3⁺ thymocytes in recipients of Maml1^−/− compared with Maml1^+/− progenitors (representative examples). Numbers indicate the percentage of cells in each box. Host-derived CD45.1⁺ cells were excluded from the analysis. (B) Absolute number of donor-derived CD3⁺ γδ T cells in the thymus of all the fetal liver chimeras that were analyzed (Maml1^+/−, ▵, n = 21; Maml1^−/−, ▴, n = 9). The increased number of γδ T cells in Maml1^−/− compared with Maml1^+/− recipients was statistically significant (P < .05; Student t test).