Figure 4
Figure 4. Normal mast-cell progenitors arise from GMPs rather than MEPs. (A) Proportion of progenitor cell types generated from 10-day liquid cultures of GMPs, CMPs, or MEPs from SCL+/Δ or SCL−/Δ mice. Wells containing only single colonies were analyzed by morphology and flow cytometry. At least 20 colonies were analyzed for each cell type. (B) Wright-Giemsa stain of a single colony from SCL+/Δ GMP culture. (C) Flow cytometric analysis of a single colony from SCL+/Δ GMP culture. (D) Wright-Giemsa stain of a single colony from a SCL+/Δ CMP culture.

Normal mast-cell progenitors arise from GMPs rather than MEPs. (A) Proportion of progenitor cell types generated from 10-day liquid cultures of GMPs, CMPs, or MEPs from SCL+/Δ or SCL−/Δ mice. Wells containing only single colonies were analyzed by morphology and flow cytometry. At least 20 colonies were analyzed for each cell type. (B) Wright-Giemsa stain of a single colony from SCL+/Δ GMP culture. (C) Flow cytometric analysis of a single colony from SCL+/Δ GMP culture. (D) Wright-Giemsa stain of a single colony from a SCL+/Δ CMP culture.

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