Elevated expression of GATA-2 in SCL^+/Δ CMPs and MEPs. (A) Relative expression of PU.1, GATA-1, and GATA-2 mRNA in GMPs, CMPs, or MEPs isolated from wild-type (+/+), SCL-heterozygous (+/Δ), and SCL-deleted (−/Δ) mice and measured by real-time PCR. Expression was normalized for HPRT and expression of the gene in SCL^+/+ CMP cells was arbitrarily set at 1.0. (B) Relative expression of GATA-2 in progenitor cells isolated on the basis of PU.1^gfp and FcRIII/II expression as described in Figure 3D. Expression was normalized for HPRT and expression of GATA-2 in SCL^+/Δ R2 cells was arbitrarily set at 1.0.