GA inhibits TNF-induced expression of NF-κB–dependent antiapoptotic, proliferative, and metastatic proteins. (A) GA inhibits the expression of TNF-induced antiapoptotic proteins. KBM-5 cells were incubated with 1.0 μM GA for 4 hours and then treated with 1 nM TNF for the indicated times. Whole-cell extracts were prepared and analyzed by Western blotting with the indicated antibodies. (B,C) GA inhibits TNF-induced expression of c-Myc, cyclin D1, COX-2, ICAM-1, MMP-9, and VEGF. KBM-5 cells were incubated with 1.0 μM GA for 4 hours and then treated with 1 nM TNF for the indicated times. Whole-cell extracts were prepared and analyzed by Western blotting with the relevant antibodies.