Local production of key inflammatory chemokines and cytokines in the lung is normal to increased in G-CSFR^−/− mice following P aeruginosa infection. The concentration of (A) G-CSF, (B) TNF-α, (C) IL-6, (D) MIP-2, and (E) KC in BALF was measured by ELISA (n = 4-7 for each time point). *P ≤ .05 compared with wild-type mice. (F) Blood monocytes isolated from wild-type mice were stimulated with LPS (10 μg/mL) for 24 hours in the presence (▪) or absence (□) of 100 ng/mL G-CSF (n = 6). TNF-α in the supernatant of these cultures was measured by ELISA. Graph shown is representative of 3 independent experiments. *P ≤ .05. All data represent the mean ± SEM.