Figure 1
Figure 1. L-α4β1 cells adhere to Lu-Fc in a divalent cation dose-dependent manner under static conditions. (A) Lu-Fc or fibronectin (Fn) (1 μg) were adsorbed onto flat-bottomed 96-well microtiter plates and 105 L-WT or L-α4β1 cells were added in the presence or absence of 1 mM EDTA and incubated for 40 minutes. (B) Adhesion assay using L-α4β1 cells and increasing amounts of Lu-Fc protein. (C) Adhesion assay using 0.5 μg of Lu-Fc and control L-WT, L-αLβ2, and L-αMβ2 cells. Error bars represent SD.

L-α4β1 cells adhere to Lu-Fc in a divalent cation dose-dependent manner under static conditions. (A) Lu-Fc or fibronectin (Fn) (1 μg) were adsorbed onto flat-bottomed 96-well microtiter plates and 105 L-WT or L-α4β1 cells were added in the presence or absence of 1 mM EDTA and incubated for 40 minutes. (B) Adhesion assay using L-α4β1 cells and increasing amounts of Lu-Fc protein. (C) Adhesion assay using 0.5 μg of Lu-Fc and control L-WT, L-αLβ2, and L-αMβ2 cells. Error bars represent SD.

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