Figure 2
Figure 2. L-α4β1 cells adhere to Lu-Fc under flow conditions. Lu-Fc (0.3 mg/mL) (A), VCAM-1-Fc (0.1 mg/mL) (B), or ICAM-1-Fc (0.3 mg/mL) (C) were immobilized on a microslide. A cell suspension including L-α4β1 and calcein-labeled L-WT or L-αLβ2 cells (107 cells/mL; 1:1) was injected in the microslide at shear stress of 0.2 dyne/cm2 for 10 minutes. Washouts (5 minutes) were applied sequentially from 0.4 to 4 dyne/cm2, and cells were counted in 6 different areas along the microslide centerline after each wash (**P < .01). Error bars represent SD.

L-α4β1 cells adhere to Lu-Fc under flow conditions. Lu-Fc (0.3 mg/mL) (A), VCAM-1-Fc (0.1 mg/mL) (B), or ICAM-1-Fc (0.3 mg/mL) (C) were immobilized on a microslide. A cell suspension including L-α4β1 and calcein-labeled L-WT or L-αLβ2 cells (107 cells/mL; 1:1) was injected in the microslide at shear stress of 0.2 dyne/cm2 for 10 minutes. Washouts (5 minutes) were applied sequentially from 0.4 to 4 dyne/cm2, and cells were counted in 6 different areas along the microslide centerline after each wash (**P < .01). Error bars represent SD.

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