Figure 3
Adhesion of L-α4β1 cells to Lu-Fc is specifically inhibited by anti-α4β1 antibodies. L-α4β1 cells (107 cells/mL) were either untreated or incubated with 10 μg/mL blocking antibodies anti–integrin α4 (HP2/1) or anti–integrin β1 (mAb 13), or isotype-matched control antibodies anti–integrin α3 (ASC-1) or anti–integrin α6 (GoH3) for 15 minutes, then injected into a Lu-Fc–coated microslide (0.3 mg/mL) for 10 minutes at 0.2 dyne/cm2. Washouts (5 minutes) were applied sequentially from 0.4 to 4 dyne/cm2, and cells were counted in 6 different areas along the microslide centerline after each wash (**P < .01). Error bars represent SD.
