Vav is differentially required for activation of LPS-induced kinase cascades. WT and Vav^null BMDMs were stimulated with LPS (10 μg/mL) and lysed at the indicated time points. Lysates were separated by SDS-PAGE and analyzed by Western blotting for (A) phosphorylated Akt (p-Akt) and total Akt, (B) phosphorylated ERK1/2 (p-ERK1/2) and total ERK2, (C) phosphorylated JNK1/2 (p-JNK1/2) and total JNK2, and (D) IκBα. Results are representative of 3 or more experiments for each protein analyzed. Densitometry was performed to quantitate the indicated phosphorylated protein relative to total levels of the protein (A-C). In addition, total levels of IκBα were quantitated by densitometry (D).