D^b-SAP tetramers eliminate most cognate CD8⁺ T cells in vitro within 72 hours. (A) Representative dotplots show the time course of deletion of female H-Y T cells incubated with hyC2A-SAP. (B). Kinetics of D^b-SAP tetramer-mediated deletion of H-Y (top) and P14 (bottom) T cells. Identical results were obtained in 3 independent experiments.