Phenotype of patient CD3ε⁺ cells. Patient and healthy volunteer PBMCs were stained and analyzed by 4-color flow cytometry. Data were collected on CD14⁻CD45⁺ lymphocytes. Shown are 2-color plots of cells stained with PerCP-labeled anti-CD3ε plus anti-TCRαβ-FITC (top row 1), PerCP-labeled anti-CD3ε plus anti-TCRγδ-PE (row 2), anti-CD3ε-APC and anti-CD4-FITC (row 3), or anti-CD3ε-APC and anti-CD8-PerCP (row 4). The 2-color plots in bottom row 5 depict anti-CD4-FITC and anti-CD8-PerCP staining on software-gated CD3ε⁺ cells. The selected gate is shown in rows 3 and 4 and was based on anti-CD3ε-APC staining intensity. Numbers represent the frequency of cells present in the indicated quadrant.