Confirmation of microarray results using TaqMan real-time PCR. Values for both cDNA array measurements (⊡) and real-time PCR (▪) are plotted as Log₂ (fold change). Results are shown as the average for the 5-paired MK/EB comparisons. Although all transcripts were detected by RT-PCR in all 5 of the MK samples, transcripts marked with an asterisk (*) were not detected in all of the EB cultures. Thus, the ratio is based on fewer than 5 comparisons (G6f, n = 2; TMSF15, n = 1; LST1 and SUCNR1, n = 4; all others n = 5). **LRRC32 was identified as differentially expressed in preliminary array experiments and by Taqman real-time PCR. Error bars represent SD of replicates.