AML cases with silenced CEBPA in cluster no. 4 simultaneously express myeloid- and T-lymphoid–specific genes and lineage markers. (A) Pairwise correlations between samples are displayed as explained in the legend to Figure 1, and GEP cluster no. 4 is enlarged in the box on the right. Histograms next to each patient display expression of selected genes with significantly elevated expression in cluster no. 4 cases with silenced CEBPA. Expression levels for probe sets of the following genes are visualized: CD7, CD3D, CD3Z, CD3G, LCK, TRD@, and NOTCH1. Corresponding expression levels and Affymetrix probe set identifiers are depicted in Table S4. (B) Representative dot plot images from flow cytometric analysis of samples obtained from 2 individual patients (ie, patients no. 2238 and no. 3491) demonstrating that the majority of cells from these patients simultaneously express CD34, CD13, CD7, and terminal deoxynucleotidyltransferase (TdT). The tumor population was identified by its weak expression of CD45, depicted in black, whereas CD45^high cells, which are considered to be mature lymphocytes, are colored in gray.