In silico engraftment kinetics depending on model parameters of donor HSCs. (A) Graphic representation of the qualitative differences of simulated engraftment kinetics depending on the choice of model parameters d and f_α(Ñ). Each individual color dot illustrates the qualitative pattern of the engraftment kinetic observed in a simulation using the particular parameter combination. In total, 55 074 different parameter combinations are shown in the figure. Simulations were initiated with one donor cell with the particular d and f_α(Ñ) values (according to the position in the diagram) and 4 competing host cells with reference parameters d = 1.07 and f_a(Ñ) = 0.01. All other parameters had been fixed to B6 reference values, identically for donor and host cells. (B) Parameter region (depicted as white square in panel A; comprising 11 682 simulation runs) that yields the best fit of the simulated to the experimentally observed distribution of engraftment kinetics (compare with Figure S2). The 2 black lines indicate the reference values for d and f_a(Ñ) used for the competing host cells. Color coding for engraftment kinetics (identical in panels A and B): maroon [+, +], red [+, π], pink [π, +], dark green [−, −], medium green [−, π], light green [π, −], purple [π,π], blue [+, −], yellow [−, +], and black (no donor engraftment).