Simulation results and experimental data on individual clone competition. (A,B) Simulated primary host engraftments of 2 clones with slightly differing parameters: (A) d = 1.0655, fα(Ñ) = 0.0075; (B) d = 1.0665, fα(Ñ) = 0.008. (C,E) 2 representative examples of secondary engraftments of the clone shown in panel A (ie, in silico transplantation of 50 ×p1 primary HSCs together with 4 residual secondary host HSCs). p is denoting the proportion of primary engraftment level, which is p1 = 0.85 in this particular example. (D,F) See panels C and E, but for the clone shown in panel B, p2 = 0.75. (G-K) A total of 4 representative examples of in silico cotransplantation of 50 × p1 cells of the clone shown in panel A plus 50 × p2 cells of the clone shown in panel B together with 4 residual secondary host cells. Further simulation examples of these settings can be found in Figure S4. (L,M) Experimentally observed engraftment kinetics (twice-monthly measurements) of 2 clonally repopulated mice. (N-Q) Engraftment kinetics for the transplantation of 5 × 106 BM cells of either one or the other clone (shown in panels L and M, respectively) in 2 lethally irradiated secondary B6 hosts. (R-U) Engraftment kinetics for cotransplantation of 2 × (5 × 106) BM cells from both primary hosts into 4 lethally irradiated secondary B6 hosts (Cho et al34 ). Color (black/gray) always illustrates relationship to primary host.
