LDI-PCR of the IGH locus in BCP-ALL with CEBP/IGH translocations. (A) Restriction enzyme map of the IGHJ region. The map was constructed based on the GenBank database (accession number NG_001019).42 Boxes represent diverse and junctional region of the IGH (D, J, respectively), IGH-specific enhancer region (Eμ), and switch region (Sμ). Arrowheads are the positions of primers for LDI-PCR. Restriction sites are as follows: H indicates HindIII; P, PstI; S, SacI; and X, XbaI. (B) Ethidium bromide–stained gel electrophoresis of LDI-PCR showing CEBP/IGH fusion genes. The LDI-PCR products representing fusions are indicated by arrowheads. An aliquot of 2 to 10 μL was loaded in each lane and electrophoresed through a 0.7% agarose gel. A DNA ladder (1 kb) was used as a molecular-weight marker.
