Effect of cerebral ischemia on LRP in the neurovascular unit. Electron microscopy analysis of LRP expression in the NVU in the ischemic (A-D) and corresponding nonischemic (E-F) hemispheres 6 hours after MCAO. Brains were stained with either the R2629 (A,C,E) or the 11H4 (B,D,F) antibodies. Arrows in panel A depict the presence of LRP-positive areas surrounding the blood vessel. Arrows in panel B point to LRP-positive perivascular astrocytes. Asterisks in panels A to D show areas where the perivascular astrocyte has detached from the basement membrane with development of early perivascular edema. Dashed arrows in panel D depict areas of astrocytic detachment of the glia limitants. Bv indicates blood vessel; bm, basement membrane; a, perivascular astrocyte; ec, endothelial cell. Magnification, ×5000 (A-B) and ×30 000 (C-F). Images were visualized using a Hitachi H-7500 electron microscope (Hitachi, Tokyo, Japan).