Effect of RAP or anti-LRP antibodies on the shedding of LRPs ectodomain, locomotor activity, and cerebral edema following MCAO. (A) Number of LRP-positive vascular structures in the 3 areas of interest in the ischemic hemisphere of wild-type animals 24 hours after MCAO and treatment with either PBS, the receptor-associated protein (RAP), or goat anti-LRP IgG. Error bars describe standard error of the mean; n = 6; *P < .05 relative to the PBS-treated group. (B) Mean locomotor activity at 6, 24, and 48 hours after MCAO and the intraventricular administration of either PBS (○), RAP (▪), or goat anti-LRP IgG (▵). Error bars describe standard error of the mean; n = 6; *P < .05 when RAP- and LRP-treated animals are compared with PBS-treated mice. (C) Evans blue dye extravasation in the same group of animals of panel B, 48 hours after MCAO. Error bars describe standard error of the mean; n = 6; *P < .05 relative to PBS-injected brains. The pictures correspond to one representative brain in each experimental group. The blue staining is apparent in areas with increase in blood-brain barrier permeability (arrows).