Figure 7
Figure 7. LRP shedding from murine astrocytes exposed to OGD conditions. (A) Representative immunoblot with the R2629 antibody of cell lysates and conditioned media of astrocytes from wild-type and tPA deficient (tPA−/−) mice exposed to either normoxic or OGD conditions for 6 hours. Top panel shows LRP in the conditioned media, and lower panel represents LRP in cell lysates. Each observation was repeated 4 times. Bar graph describes mean density of the band per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. †P < .001 and ¶P < .05 relative to astrocytes exposed to normoxic conditions (B) Representative immunoblot with the R2629 antibody of cell lysates and conditioned medium of astrocytes from wild-type mice exposed to either normoxia or OGD conditions, in the presence or absence of either tPA or tPA and RAP. Upper panel shows LRP in the conditioned media and lower panel represents LRP in cell lysates. Each observation was repeated 4 times. Bar graph describes mean density of the band per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. ±P < .01 and ¶P < .05 relative to astrocytes exposed to normoxic conditions in absence of tPA. £P < .01 relative to astrocytes exposed to OGD conditions without tPA or with tPA plus RAP. *P < .05 relative to astrocytes exposed to OGD conditions in the absence of tPA. (C) Representative immunoblot with the R2629 antibody of cell lysates and conditioned media of astrocytes from wild-type mice exposed to either normoxia or OGD conditions, in the presence or absence of RAP. Each observation was repeated 4 times. Bar graphs describe mean density of the band for a total of 4 observations per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. ⋀P < .01 relative to astrocytes maintained under normoxic conditions; ‖P < .05 relative to astrocytes exposed to OGD conditions in the absence of RAP.

LRP shedding from murine astrocytes exposed to OGD conditions. (A) Representative immunoblot with the R2629 antibody of cell lysates and conditioned media of astrocytes from wild-type and tPA deficient (tPA−/−) mice exposed to either normoxic or OGD conditions for 6 hours. Top panel shows LRP in the conditioned media, and lower panel represents LRP in cell lysates. Each observation was repeated 4 times. Bar graph describes mean density of the band per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. †P < .001 and ¶P < .05 relative to astrocytes exposed to normoxic conditions (B) Representative immunoblot with the R2629 antibody of cell lysates and conditioned medium of astrocytes from wild-type mice exposed to either normoxia or OGD conditions, in the presence or absence of either tPA or tPA and RAP. Upper panel shows LRP in the conditioned media and lower panel represents LRP in cell lysates. Each observation was repeated 4 times. Bar graph describes mean density of the band per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. ±P < .01 and ¶P < .05 relative to astrocytes exposed to normoxic conditions in absence of tPA. £P < .01 relative to astrocytes exposed to OGD conditions without tPA or with tPA plus RAP. *P < .05 relative to astrocytes exposed to OGD conditions in the absence of tPA. (C) Representative immunoblot with the R2629 antibody of cell lysates and conditioned media of astrocytes from wild-type mice exposed to either normoxia or OGD conditions, in the presence or absence of RAP. Each observation was repeated 4 times. Bar graphs describe mean density of the band for a total of 4 observations per experimental group. Lines depict SEM. White bars represent results obtained under normoxic conditions. Black bars depict observations made under OGD conditions. ⋀P < .01 relative to astrocytes maintained under normoxic conditions; ‖P < .05 relative to astrocytes exposed to OGD conditions in the absence of RAP.

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