Characteristic profile of DCregs. (A-C) The expression of cell surface molecules on mDCs (A,C) and DCregs (B,C) was analyzed by flow cytometry, and data are represented by a dot plot (A,B) and mean fluorescence intensity (C). The results are representative of 4 experiments with similar results. The error bars indicate SD. *P less than .01 compared with mDCs. (D) DCs (5 × 105) were stimulated or not stimulated with CpG ODN (0.1 μM) for 24 hours, and the culture supernatants were analyzed for cytokine production. The results are representative of 4 experiments with similar results. The error bars indicate SD. *P less than .01 compared with mDCs.