Characterization of human CMP- and CLP-derived DC subsets. Flow-cytometric analysis of surface DC–related antigens in human CMP-derived and human CLP-derived cDCs (A) and pDCs (B). The gray area represents each isotype control. Note that the expression patterns of each DC-related antigen are almost identical in cDCs and pDCs, irrespective of their CMP or CLP origin. (C) The relative expression levels of RNAs isolated from cDCs and pDCs in the bone marrow of mice that received CMPs (top) and CLPs (middle) and in normal human bone marrow (bottom). Representative data from 3 independent experiments using real-time PCR analyses are shown. Error bars indicate the SEM of triplicate cultures.