CXCR3 expression on naive, memory, and regulatory T-cell subsets from SCT patients with and without GVHD. CXCR3 expression on naive (CCR7⁺CD45RA⁺), central memory (CCR7⁺CD45RA⁻), effector memory (CCR7⁻CD45RA⁻), and effector memory revertant (CCR7⁻CD45RA⁺) CD4⁺ (A) and CD8⁺ (B) T cells from SCT patients with GVHD (▨) and without GVHD (□). The y-axis represents the proportion of T cells positive for CXCR3. Bars represent median and range. (C) Regulatory T-cell numbers in PBMCs. PBMCs were gated on CD4⁺CD45RO⁺ T cells and T reg frequency was determined by their positive expression for CD25 and low expression for CD127. (D) CXCR3 expression on T reg cells. ▴ indicates SCT patients with GVHD; ▵ indicates control SCT patients without GVHD. The y-axis represents the number of cells positive for marker(s) per microliter of peripheral blood. Solid line represents the mean.