American Society of Hematology

Figure 3

$Figure 3. Sessile and BM-derived KCs have equal phagocytic capacity. To evaluate the phagocytic capacity of BM-derived and sessile KCs, radiation bone marrow chimeras (10-Gy radiation dose) were injected with fluorescent beads, which underwent rapid phagocytosis by macrophages. (A, left) The distribution of fluorescent beads in BM-derived and sessile KCs was assessed by costaining with a fluorescent anti-CD45.1 mAb. Double-positive CD45.1 KCs appeared purple (closed arrow), whereas sessile (CD45.2+) KCs only stained with F4/80 (red, open arrow). Original magnification, ×200 (left). Higher magnification, ×400 (right). (B) Quantitative analysis of bead-carrying KCs revealed an almost equal distribution of phagocytic BM-derived and sessile KCs (52% ± 1.7% vs 48% ± 1.7%). (C) Phagocytic activity of BM-derived (26% ± 1.7%) versus sessile KCs (32% ± 5.3%) expressed as a fraction of the total KCs.$

Sessile and BM-derived KCs have equal phagocytic capacity. To evaluate the phagocytic capacity of BM-derived and sessile KCs, radiation bone marrow chimeras (10-Gy radiation dose) were injected with fluorescent beads, which underwent rapid phagocytosis by macrophages. (A, left) The distribution of fluorescent beads in BM-derived and sessile KCs was assessed by costaining with a fluorescent anti-CD45.1 mAb. Double-positive CD45.1 KCs appeared purple (closed arrow), whereas sessile (CD45.2⁺) KCs only stained with F4/80 (red, open arrow). Original magnification, ×200 (left). Higher magnification, ×400 (right). (B) Quantitative analysis of bead-carrying KCs revealed an almost equal distribution of phagocytic BM-derived and sessile KCs (52% ± 1.7% vs 48% ± 1.7%). (C) Phagocytic activity of BM-derived (26% ± 1.7%) versus sessile KCs (32% ± 5.3%) expressed as a fraction of the total KCs.

This Feature Is Available To Subscribers Only