Effect of anti-H2B and antienolase Fab on macrophage recruitment in response to TG. (A) Fab (500 μg in 200 μL PBS) was injected intravenously into C57BL/6 mice 15 minutes prior to intraperitoneal injection of TG. Another dose of each Fab was injected intravenously at 24 hours. Peritoneal lavage was collected into cold sterile HBSS, and the cells were collected by centrifugation, washed with HBSS-HEPES, and lysed in 1% TritonX. The number of macrophages in the lavage was determined using nonspecific esterase activity (n = 3). ■ indicate mice treated with nonimmune Fab; ▧, mice treated with anti-H2B Fab. Significance was calculated by a Student t test. (B) C57BL/6 mice were injected intravenously with Fab to C-terminus and N-terminus H2B, α-enolase, or nonimmune Fab, 15 minutes prior to TG administration. Lavage cells (from 2-mL lavage) were collected at 72 hours and macrophages were quantified as in panel A.