The Lycat transgene increased protein expression of hematopoietic and endothelial cells, as detected by flow cytometry. The Lycat transgenic and vector control ES cell clones were induced into EBs at day 4 to day 6. Expression of Flk1 (A), CD41 (B), CD41 and C-Kit (C), and CD31 (E) was increased in D4 EBs and expression of CD45 (D) was increased in D6 EBs from transgenic ES cells (FC5 and FC22). IgG indicates negative controls of FACS without input of the primary antibody. These quantitative effects were calculated from 3 independent experiments. Numbers within quadrants indicate percentages of positive cells.