Cdc42 deletion affected hematopoietic progenitor populations. (A) Immunophenotypic analysis of various progenitors in WT and KO bone marrow. Representative FACS staining profiles of the progenitor populations, including LSKs, LKs, CMPs, MEPs, and GMPs, from the respective bone marrow of WT or KO mice at 14 DPI are shown. The gating is described in “Hematologic analysis and flow cytometry.” (B) The numbers and percentages of LSK, LK, CMP, MEP, and GMP populations in the bone marrow of WT and KO mice are calculated according to the phenotype determined by flow cytometry shown in panel A. Values were derived from 6 mice of each genotype. *P < .05; **P < .01; and ***P < .001.