Cdc42 deletion results in altered transcription factor network in progenitors. (A) The relative Cdc42-GTP level in the WT low-density bone marrow cells under various stimulatory conditions was determined by the GST-PAK1 effector domain pull-down assay as described in “Cdc42 activity effector domain pull-down assay.” (B) Changes in gene transcription of purified LSK, CMP, GMP, and MEP cells from WT and KO bone marrow were measured by quantitative real-time PCR. The relative transcript levels of each cell population were internally normalized to that of GAPDH, and the results are expressed as (= KO/WT − 1). Each cell population was pooled from at least 4 mice and values are representative of at least 2 independent experiments performed in triplicate. *P < .05; **P < .01; and ***P < .001.