LTC₄ and PGD₂ generation in response to Pam₃CSK₄ is attenuated in Pla2g5–null BMMCs. (A-B) BMMCs from wild-type (shaded bars) and Pla2g5-null (open bars) mice were incubated for 60 minutes with the indicated concentrations of Pam₃CSK₄. (C-D) BMMCs from wild-type (shaded bars) and Pla2g5-null (open bars) mice were incubated for 0 to 60 minutes with 3 μg/mL Pam₃CSK₄ or with medium alone (M) as a control. Supernatants were assayed for LTC₄ (A,C) and PGD₂ (B,D) by EIA. The values are the mean ± SEM of results from 2 independent experiments obtained from 6 separate cultures of both wild-type and Pla2g5-null BMMCs (n = 6). *P < .05, +P < .01, **P < .001, by Student t test in posthoc analyses after ANOVA.