p80HT transgenic mice develop disseminated B or T cell lymphomas. (A) Histopathologic analysis of lymphomas in p80HT mice by hematoxylin and eosin staining. The normal architecture shown in the wild-type organs are completely effaced by extensive lymphocyte infiltrations in the organs from a dead p80HT mouse. (B) Immunohistochemical examination of p80HT mouse lung sections with malignant lymphocyte infiltration. The sections stained strongly either for B220 (a B cell marker) or CD3 (a T cell marker), indicative of B or T cell lymphomas. Scale bars in panels A and B, 50 μm. (C) Southern blot analysis of IgH (top) and TCR (bottom) gene rearrangements in representative lymphoma samples. EcoRI-digested DNA was hybridized with an IgH-μ JH4 probe or with a TCR Cβ1 probe. Tail DNA from a p80HT mouse was used as control for the germ line (GL) IgH and TCR loci. Arrowheads indicate rearrangements at either the IgH-μ or the TCR-β locus. Size markers in kilobases are shown to the right.
