p80HT up-regulates TRAF1 expression. (A) Immunoblot analysis of the expression of indicated antiapoptotic genes in splenic B cells from wild-type (WT) and p80HT mice and in representative B lymphoma samples. Levels of α-tubulin are shown as loading control. (B) Immunoblot analysis of TRAF1 expression in splenic T cells from wild-type (WT) and p80HT mice and in one T lymphoma sample. Levels of α-tubulin are shown as loading control. (C) Immunoblot analysis of TRAF1 and XIAP expression in the human fibrosarcoma HT1080 cells infected with GFP- or p80HT-expressing retroviruses, the T-cell leukemia cell line Jurkat, and the T-cell lymphoma cell line HUT78 that harbors a mutated NF-κB2 allele encoding p80HT. Levels of α-tubulin are shown as loading control. (D) Ribonuclease protection assay for mRNA levels of the indicated antiapoptotic genes in the same human cell lines. The mRNA levels for the housekeeping genes L32 and GAPDH are shown as loading control.