Cell adhesion–mediated down-regulation of Skp2 requires Cdh1. (A) The Cdh1 expression in Jeko-1 cells in suspension (Sus) versus HS-5 adhesion (HS5-Ad) analyzed by Western blot. Cell-cycle distribution (B) and protein levels of p27^Kip1, Skp2, and Cdh1 (C) 24 hours after transfection with Skp2 siRNA (siSkp2), Cdh1 siRNA (siCdh1), or control siRNA (siCont) in Jeko-1 lymphoma cells in suspension. All data are representative of at least 3 experiments (means ± SD).