TSLP Tg expression rescues B- and T-cell development in IL-7^−/− mice. IL-7^−/−, WT, and IL-7^−/− K14-TSLP Tg mice were analyzed at 12 weeks of age. (A) Absolute cell number per 2 femurs. (B) Regions indicate the pro-B/pre-B-I (CD19⁺ c-kit⁺), pre-BII (CD19⁺ CD25⁺), mature (CD19⁺ B220^high IgM⁺), and immature (CD19⁺ B220⁺ IgM⁺) B cells in the BM. Numbers are mean and standard deviation of percentage (n = 5). (C) Absolute thymocyte number and (D) thymocyte profiles of DN1 (c-kit^high CD44⁺ CD25⁻), DN2 (c-kit^high CD44⁺ CD25⁺), DN3 (CD44⁻ CD25⁺), DN4 (CD44⁻ CD25⁻), DP (CD4⁺ CD8⁺), and SP (CD4⁺ CD8⁻, CD4⁻CD8⁺) thymocytes as well as of immature (CD24^lo/− γδ⁺) and mature (CD24⁺ γδ⁺) γδ T cells are shown. (E) Pregated on CD4⁻ CD8⁻ cells, percentages of Vγ2 and Vγ1.1 T cells in the thymus are shown. (F) Pregated on CD45⁺ cells, percentages of Vγ3⁺ CD3⁺ cells in the skin are shown. Histograms represent the mean and standard deviation from analyzing 5 animals.