Correlation between AnV and CD45RO expression levels. Total tonsillar lymphocytes were stained for GC and naive B-cell subsets as before with the addition of anti-AnV–APC. (A) RO versus AnV contour plots are shown for each population, including the APC-isotype control. The total percentage of AnV⁺ cells is indicated. *Distribution of AnV⁺ cells across the RO spectrum (ie, 94.5% of all AnV⁺ GC B cells [0.6%] are RO⁻ compared with only 0.5% of RO⁺ GC cells). The proximity of most AnV⁺ cells to the RO⁻/RO^+/− boundary suggests the RO⁻ fraction can be further subdivided, highlighting an RO^−/lo fraction. (B) CD69 MFIs were calculated for AnV⁻ (□) and AnV⁺ (■) cells within each RO fraction. In the case of RO⁺ where there was a dearth of AnV⁺ cells, comparisons were made by gating on the highest and lowest 5% of cells under the AnV bell curve. × denotes no data point.