American Society of Hematology

Figure 5

$Figure 5. Correlation between GC B-cell CD45RO expression and activation markers CD69, CD71, and Ki67. (A) GC B cells were costained with antibodies against RO and a panel of activation markers, including CD69, CD71, and Ki67. Each activation marker generates a cytometric bell curve distribution. Therefore, isotype controls were used to demarcate positive and negative gates (dashed lines). Quadrant classification follows the standard Cartesian system such that RO is tri-sected into 3 expression levels (−, +/−, and +). All other markers are bisected into a “−” and “+” quadrant. The propensity of cell proliferation is indicated by a CD71-versus-Ki67 plot (greatest among CD71+Ki67+ double-positive population). (B) CD69, CD71, and Ki67 MFIs were calculated for each GC B-cell RO fraction (RO−, □; RO+/−, ▩; and RO+, ■).$

Correlation between GC B-cell CD45RO expression and activation markers CD69, CD71, and Ki67. (A) GC B cells were costained with antibodies against RO and a panel of activation markers, including CD69, CD71, and Ki67. Each activation marker generates a cytometric bell curve distribution. Therefore, isotype controls were used to demarcate positive and negative gates (dashed lines). Quadrant classification follows the standard Cartesian system such that RO is tri-sected into 3 expression levels (−, +/−, and +). All other markers are bisected into a “−” and “+” quadrant. The propensity of cell proliferation is indicated by a CD71-versus-Ki67 plot (greatest among CD71⁺Ki67⁺ double-positive population). (B) CD69, CD71, and Ki67 MFIs were calculated for each GC B-cell RO fraction (RO⁻, □; RO^+/−, ▩; and RO⁺, ■).

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