Frequencies and phenotype of HLA-G^pos T cells in peripheral blood. (A) FACS staining of peripheral blood lymphocytes from healthy donors for HLA-G on CD4 and CD8 lymphocytes. Gates for CD4 and CD8 T cells were set on FSC/CD4 and FSC/CD8 dot blots to identify the desired subsets. HLA-G expression (bottom panel) was assessed in comparison with the control isotype staining (top panel). A representative staining is shown. (B) Percentages of HLA-G^pos T cells in the CD4⁺ or CD8⁺ subpopulations of healthy volunteers (mean and SD).