Mobilization of SKL and CD34⁻ SKL cells in mice mobilized with G-CSF or GRO. (A) Lin⁻ PBMC were stained with anti-c-kit, anti-Sca-1, and anti-CD34. The upper plots show isotype (left) and CD34 and lineage marker staining (right). The R1 gate represents CD34⁻ lin⁻ cells. The bottom left plots show the isotype (left) and c-kit and Sca-1 staining in CD34⁻ lin⁻ cells (right) in the R1 gate. The R2 gate represents c-kit⁺ Sca-1⁺ cells. The plot shown is representative data acquiring approximately 0.5 × 10³ SKL events in the CD34⁻ lin⁻ cell fraction. (B) SKL cells (left panel) and CD34⁻ SKL cells (right panel) in 2 × 10⁶ PBMCs. Data are expressed as mean (± SEM) from 6 replicates of 10 mice per group in 2 experiments. †P < .05 compared with G-CSF; ‡Synergy compared with G-CSF or GROβ_Δ4, P < .05, determined using ANOVA with Bonferroni multiple comparison test.