EKLF expression during hematopoietic cell differentiation. Cell populations were sorted as detailed in Table S1 and monitored for EKLF expression by quantitative RT-PCR. Two experiments are shown (A,B) and the results summarized (C; pathway based on Weissman et al² ). Each sample was monitored in triplicate; values were normalized in each experiment to the EKLF level in megakaryocyte/erythroid progenitors (MEPs) which was set to 100. LT-HSC indicates long-term hematopoietic stem cell; MPP, multipotent progenitor; CMP, common myeloid/erythroid progenitor; GMP, granulocyte/macrophage progenitor; MEP, megakaryocyte/erythroid progenitor; CLP, common lymphoid progenitor; ProT1 and ProT2, T-cell progenitor; proB, B-cell progenitor; EP, erythroid progenitor; MkP, megakaryocyte progenitor; CMP F+, myeloid and B-cell progenitor; CMP F−, myeloid/erythroid progenitor; Mo, monocytes; N, neutrophile granulocytes; Ma, bone marrow derived macrophages; DC, dendritic cells.