Expression of selected genes after EKLF induction during EB differentiation. Day 6 EBs (EBd6) were untreated (−) or treated (+) with doxycycline for 24 (Mkd7) or 48 (Mkd8) hours during growth on OP9 cells in TPO as in Figure 3. Total RNA was isolated from each, and the same sample was monitored for expression of EKLF, Fli-1, and β maj (A) or GATA1, GATA2, and RUNX1 (B). Expression normalized to levels of GAPDH is graphed; all analyses were performed in triplicate, and panel A was from 2 independent experiments. Error bars represent SD.