Cells transformed by FTKs display enhanced expression and phosphorylation of Nbs1. (A) Mre11, Nbs1, and RAD50 expression was examined by Western blot analysis in total cell lysates from Baf3 (P) or Baf3 cells expressing the following FTKs: BCR/ABL (B/A), TEL/JAK2 (T/J), BCR/FGFR (B/F), NPM/ALK (N/A), TEL/ABL (T/A), TEL/PDGFβR (T/P), and TEL/TRKC(L) (T/T). Cells were starved from IL-3 for 12 hours. (B) pNbs1 and Nbs1 expression was examined in the untreated and MMC-treated (0.5 μg/mL for 12 h) cells in the presence of IL-3. Tubulin was detected as a loading control.