Bad- or Bid-deficiency did not further enhance the abnormal accumulation of antigen-specific B cells in bim^−/− mice. Mice (wt, bim^−/−, bim^−/−bad^−/−, or bim^−/−bid^−/−) were injected intraperitoneally with 100 μg of NP coupled to KLH, and leukocytes were collected from the spleen after 7, 14, and 28 days. Cells were stained with fluorochrome-conjugated surface marker-specific monoclonal antibodies and gated on IgM⁻IgD⁻Gr-1⁻Mac-1⁻ cells by flow cytometry and analyzed for their proportion of antigen-specific B220⁺IgG1⁺NP⁺ B cells as illustrated in Figure 1A. The total numbers of antigen-specific IgG1⁺NP⁺ B cells in the spleen of bim^−/−bad^−/− and bim^−/−bid^−/− (A) were determined, and their percentages in the peripheral blood are also shown (B). Each data point represents a mouse: n = 2-3 bim^−/−bad^−/− mice, n = 2 bim^−/−bid^−/− mice, n = 3 bim^−/−, and n = 3 wt mice.