DEX-induced histone H4 K5 acetylation in human CD4⁺ and CD8⁺ T cells. Representative images of acetylated histone H4 K5 in CD4⁺ and CD8⁺ cells in response to 6 hours of 10⁻⁷ M DEX treatment in vitro are shown (original magnification × 630; blue, DAPI, nuclear staining; red, cy3, acetylated histone H4 K5; green, APC, surface staining with (A) anti-CD4 or (C) anti-CD8 APC antibodies). The MFI of cy3 staining (acetylated histone H4 K5) in CD4⁺ (B) and CD8⁺ (D) cells was assessed by analysis software within the computer-generated masks for the cell nuclei. Cells (50–100) were analyzed for each donor studied (n = 8). See “Materials and methods, GCRα nuclear translocation” for more detailed image acquisition information.