HLA-G affects both CD4 and CD8 gene transcription and expression. T cells were primed either with APCs or APC-HLA-G1s or with HLA-G5⁻ or HLA-G5⁺ SNs for 6 days. T cells and supernatants were then collected for further analysis. (A,B) Total RNA was isolated from T-cell populations and used for real-time RT-PCR analysis using specific CD4 (A) or CD8 (B) primer and probe. Relative quantities of CD4 (A) and CD8 (B) transcripts in each T-cell population are shown in comparison with those of control healthy donor's PBMCs (assigned a value of 1). Results are expressed as mean plus or minus SEM from 4 independent experiments. (C,D) Levels of soluble CD4 (C) and CD8 (D) molecules were measured by specific ELISA in supernatants from T cells primed either with APCs or APC-HLA-G1s or with HLA-G5⁻ or HLA-G5⁺ SNs. Concentrations are given as mean plus or minus SEM.