memTNF T cells induce less GVHD mortality and morbidity than wt T cells and have intact GVT activity. (A) Lethally irradiated (850 cGy) BALB/c mice received 5 × 106 TCD wt B6 BM cells alone (n = 5) or in combination with 0.5 × 106 (n = 20) or 1 × 106 (n = 10) wt or memTNF B6 T cells. Data represent 2 combined experiments. (B) Lethally irradiated (1300 cGy) C3FeB6F1 mice received 5 × 106 TCD wt B6 BM cells alone (n = 9) or in combination with 1 × 106 purified CD4+ T cells from wt or memTNF B6 mice (n = 18). Data represent 2 combined experiments. (C) Lethally irradiated (850 cGy) BALB/c mice received 5 × 106 TCD wt B6 BM cells and 1 × 106 A20 lymphoma cells (n = 10) with or without the addition of 0.5 × 106 B6 wt or memTNF B6 T cells (n = 20). Survival was monitored daily and cause of death (tumor versus GVHD) was determined by necropsy and histopathology. Data represent 2 combined experiments. N/A indicates not analyzed due to tissue necrosis. (D) Lethally irradiated (1300 cGy) B6D2F1 mice received 5 × 106 TCD wt B6 BM cells and 1 × 103 P815 mastocytoma cells (n = 21) with or without the addition of 0.5 × 106 B6 wt (n = 20) or memTNF B6 T cells (n = 22). Survival was monitored daily and cause of death (tumor versus GVHD) was determined by necropsy and histopathology. Data represent 3 combined experiments.
