Changes in NCRs (NKp30, NKp44, and NKp46) and NKG2D after culture with CSA. (A) NK cells were cultured in IL-2 (100 U/mL) and IL-15 (10 ng/mL) with and without CSA for 7 days and analyzed by FACS. NKp30 staining for cells from a representative donor is shown. gMFI is listed in right lower portion of figure. (B) Relative change in gMFI for NKp30, NKp44, NKp46, and NKG2D after culture with CSA. Relative change in gMFI = 100 × (gMFI of receptor in CSA − gMFI of receptor in control)/gMFI of receptor in control. Results are the average of samples from 16 healthy donors. Statistics were calculated using the Wilcoxon signed rank test.