Isolation, self-renewal, and differentiation of the ROS^low and ROS^high subsets. (A) Isolation of ROS^low and ROS^high populations. ROS^low or ROS^high Lin⁻CD45⁺AnV⁻ marrow cells were isolated using DCF-DA cell-permeable intracellular ROS indicator. R2 indicates ROS^low; R5, ROS^high. (B) LTC-ICs at 6 weeks (top), CFU-Cs at 10 days (middle and bottom). (C) Competitive repopulation assay with 10 to 2500 cells. A total of 10 mice for each cell dose were used for the transplantation in 2 independent experiments. (D) Multilineage reconstitution potential (left). Myeloid differentiation skewing of the ROS^high population (right). Values represent the means plus or minus SEM. *P < .05; **P < .01.