Exhaustion of HSCs in serial transplantation of the ROS^high population and the decreased adherence to the specific niche components. Reconstitution capacity in secondary (A) and tertiary (B) transplantation. A total of 5 to 10 mice for each condition were used as recipients. (C) Cell adhesion assay. Values represent the means plus or minus SEM and are significant at *P < .05 and **P < .01.