![Figure 1. The highly conserved cytoplasmic domain of CD43 is constitutively phosphorylated in ex vivo murine T cells. (A) Purified ex vivo DO.11.10 T cells or (B) resting DO.11.10 T-cell lymphoblasts were labeled with [32P] orthophosphate, incubated in the absence or presence of PMA (50 ng/mL) for the indicated time, and lysed. CD43 was immunoprecipitated separated by SDS-PAGE, and phosphorylation was detected by autoradiography. (C) Alignment of the CD43 cytoplasmic domain from human, chimpanzee, mouse, rat, cattle, and dog using ClustalW (version 1.8).43 Numbering refers to the human sequence, with 1 indicating the start of the cytoplasmic domain. Numbering from the translational start site is indicated in parentheses. *Identical residues in all sequences in the alignment (shaded in black); “:” indicates conserved substitutions (shaded in gray), and “.” indicates semiconserved substitutions. Serine and threonine residues conserved across all species are denoted by ▾. Positions of murine Ser72 and Ser76 (corresponding to human Ser74 and Ser78) are indicated by † and ‡, respectively.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/8/10.1182_blood-2007-01-065276/4/zh80210708710001.jpeg?Expires=1736059468&Signature=IdIyhh~LcJxMdb-0-Ups6Q0Vm1jKgskyIWhNEtLmmbu8CJac8iR4wHAuU8HjP8YGl2Z-mIOktrvF~7zzLn1Glk8ChZI~F5vgO4ZeFPA3cXqZ5lNmrBEbWz5cInzvdFpQfXoNM~HNgZrSrpEkPAIYnJSi8~imxpZiC1kjtD1MbwifZ7eDulqo2yHOYwxbkSqdY1OMPHfWBboeiwK8Jy6Evlp7FRKneffAKS5bBBH83Kw8DqA31f6VQKn3-sKP-Cw~xpJi~2kO9xX0zrinVAmK-Ul3QcyTvXz3tgtzZozz4akAOKKtBSlyUEmMFWg1vOWNh1Jdw9l80qJ3QMHCVzn8RA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The highly conserved cytoplasmic domain of CD43 is constitutively phosphorylated in ex vivo murine T cells. (A) Purified ex vivo DO.11.10 T cells or (B) resting DO.11.10 T-cell lymphoblasts were labeled with [32P] orthophosphate, incubated in the absence or presence of PMA (50 ng/mL) for the indicated time, and lysed. CD43 was immunoprecipitated separated by SDS-PAGE, and phosphorylation was detected by autoradiography. (C) Alignment of the CD43 cytoplasmic domain from human, chimpanzee, mouse, rat, cattle, and dog using ClustalW (version 1.8).43 Numbering refers to the human sequence, with 1 indicating the start of the cytoplasmic domain. Numbering from the translational start site is indicated in parentheses. *Identical residues in all sequences in the alignment (shaded in black); “:” indicates conserved substitutions (shaded in gray), and “.” indicates semiconserved substitutions. Serine and threonine residues conserved across all species are denoted by ▾. Positions of murine Ser72 and Ser76 (corresponding to human Ser74 and Ser78) are indicated by † and ‡, respectively.
