LRP-1 inhibits NF-κB activation. (A) Nuclear extracts from PEA-10, MEF-2, and B-41 cells were analyzed by EMSA using an NF-κB–specific biotin-labeled probe, in the presence or absence of an excess concentration of the cold probe. (B) Nuclear extracts from CL-16 cells that express LRP-1 and from shLRP-1 cells, which are CL16 cells in which LRP-1 has been silenced with shRNA, were analyzed by EMSA for NF-κB activity. (C-E) RAW 264.7 cells were transiently transfected with NTC or LRP-1-specific siRNA. (C) Expression of LRP-1 was determined by qPCR 36 hours later. (D) Expression of C1r was determined 36 hours later. Error bars represent SD. (E) EMSA assay for active NF-κB using nuclear extracts from RAW 264.7 cells transfected with NTC or LRP-1-specific siRNA.