Cited2 is expressed in Lin⁻c-Kit⁺, Lin⁻c-Kit⁺Sca-1⁺ (LSK) fetal liver cells. Fetal liver cells from 14.5 dpc were sorted for Lin⁻c-Kit⁺ and LSK subpopulations. Sorted cells were processed for mRNA expression of Cited2 by RT-PCR, and the expression was further quantified by real-time PCR with 18S as the internal control for verifying the presence of cDNA after reverse transcription and for quantification purposes. (A) Cited2 expression was detected in Lin⁻c-Kit⁺ and LSK cells. (B) Cited2 expression in LSK cells was approximately 3-fold higher than that in Lin⁻c-Kit⁺ cells. Two sets of independently sorted cells were performed in parallel for Cited2 mRNA expression analysis.