Reduced number of LSK cells in Cited2^−/− fetal liver. Fetal liver cells from (A) Cited2^+/+ littermate control (n = 5) and (B) Cited2^−/− (n = 4) at 14.5 dpc were sorted as shown by the representative lineage and sorting gates (LSK cells were collected from Q2). Ten thousand counts were presented in panels A,B. The numbers within the quadrants represent the percentage of cells. The yield of LSK cells sorted from Q2 was too low to be reanalyzed. (C) The cumulative counts of LSK cells obtained by flow cytometer during the sorting procedure, which was compared between Cited2^−/− and Cited2^+/+ littermate control and revealed significant reduction in Cited2^−/− fetal liver (P < .01; average ± SEM).